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ptk-rl plasmids  (Thermo Fisher)


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    Thermo Fisher ptk-rl plasmids
    Ptk Rl Plasmids, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptk-rl+plasmids/pmc08938476-891-19-30
    Average 90 stars, based on 1 article reviews
    ptk-rl plasmids - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Targeting HNRNPM Inhibits Cancer Stemness and Enhances Antitumor Immunity in Wnt-activated Hepatocellular Carcinoma
    Article Snippet: Briefly, tumor cells (5 × 10 4 ) were seeded into a 24-well plate, and TOP-Flash reporter plasmids and pTK-RL plasmids were transiently co-transfected into the cells using Lipofectamine 2000 (Invitrogen).

    Article Title: LGR5 promotes the proliferation and tumor formation of cervical cancer cells through the Wnt/β-catenin signaling pathway
    Article Snippet: In brief, TOP-Flash reporter and pTK-RL plasmids were transiently co-transfected into tumor cells with Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) in 24-well plates, and the activity of both firefly and Renilla luciferase reporters was determined at 48 hours after transfection using the Dual Luciferase Assay kit (Promega, Madison, WI, USA) according to the manufacturer's instructions.

    Luciferase:

    Article Title: Targeting HNRNPM Inhibits Cancer Stemness and Enhances Antitumor Immunity in Wnt-activated Hepatocellular Carcinoma
    Article Snippet: Briefly, tumor cells (5 × 10 4 ) were seeded into a 24-well plate, and TOP-Flash reporter plasmids and pTK-RL plasmids were transiently co-transfected into the cells using Lipofectamine 2000 (Invitrogen).

    Article Title: LGR5 promotes the proliferation and tumor formation of cervical cancer cells through the Wnt/β-catenin signaling pathway
    Article Snippet: In brief, TOP-Flash reporter and pTK-RL plasmids were transiently co-transfected into tumor cells with Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) in 24-well plates, and the activity of both firefly and Renilla luciferase reporters was determined at 48 hours after transfection using the Dual Luciferase Assay kit (Promega, Madison, WI, USA) according to the manufacturer's instructions.

    Transfection:

    Article Title: Targeting HNRNPM Inhibits Cancer Stemness and Enhances Antitumor Immunity in Wnt-activated Hepatocellular Carcinoma
    Article Snippet: Briefly, tumor cells (5 × 10 4 ) were seeded into a 24-well plate, and TOP-Flash reporter plasmids and pTK-RL plasmids were transiently co-transfected into the cells using Lipofectamine 2000 (Invitrogen).

    Article Title: LGR5 promotes the proliferation and tumor formation of cervical cancer cells through the Wnt/β-catenin signaling pathway
    Article Snippet: In brief, TOP-Flash reporter and pTK-RL plasmids were transiently co-transfected into tumor cells with Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) in 24-well plates, and the activity of both firefly and Renilla luciferase reporters was determined at 48 hours after transfection using the Dual Luciferase Assay kit (Promega, Madison, WI, USA) according to the manufacturer's instructions.



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    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative <t>luciferase</t> (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by <t>Renilla</t> luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.
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    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative <t>luciferase</t> (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by <t>Renilla</t> luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.
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    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative <t>luciferase</t> (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by <t>Renilla</t> luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.
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    Image Search Results


    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative luciferase (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by Renilla luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.

    Journal: Cancer Science

    Article Title: Application of prime editing system to introduce TP53 R248Q hotspot mutation in acute lymphoblastic leukemia cell line

    doi: 10.1111/cas.16162

    Figure Lengend Snippet: Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative luciferase (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by Renilla luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.

    Article Snippet: Renilla luciferase reporter plasmid pTK‐RL (E2231; Promega) was cotransfected as the internal standard.

    Techniques: Activity Assay, Targeted Gene Expression, Reporter Assay, Luciferase, Cell Culture, Produced, Expressing, Quantitative RT-PCR